Structure of transcriptionally-active chromatin subunits
نویسندگان
چکیده
منابع مشابه
Structure of transcriptionally active chromatin.
Rat-liver chromatin has bee fractionated into transcriptionally active and inactive regions [Gottesfeld et al. (1974) Proc. Nat. Acad. Sci. USA 71, 2193-2197] and the distribution of nuclease-resistant complexes in these fractions has been investigated. About half of the DNA of both fractions is resistant to attack by tne endonuclease DNase II. The nuclease-resistant structures of inactive chro...
متن کاملStructure and dynamics of transcriptionally active chromatin.
Chromatin is a highly dynamic macromolecular complex that undergoes continuous structural modification during the various stages of genetic activity. Such dynamic behavior is the result of a complex and perfectly balanced network of interactions involving histones, DNA and ions in an aqueous environment. In the past eighteen years since the discovery of the nucleosome particle, a great deal of ...
متن کاملOrganization of Transcriptionally Active and Inactive Chromatin
Der Aufbau des Nukleosoms als Grundbaustein des Chromatins der Eukaryontenzelle, mit Ausnahme des Dinoflagellaten-Zellkerns, wird biochemisch und elektronenmikroskopisch vorgestellt als eine Einheit eines Oktamers aus Histonen (2 X H2A, 2XH2B, 2X H3, 2X H4) und einem Betrag von etwa 150 Basenpaaren DNA. Diese Partikel sind durch einen Zwischenbereich von weiteren etwa 50 Basenpaaren DNA verbund...
متن کاملStructure of active chromatin: higher-order folding of transcriptionally active chromatin in control and hypothyroid rat liver.
Investigation have been carried out into the salt-induced higher-order folding in the transcriptionally active chromatin region of rat liver nuclei by nuclease digestion, sedimentation and CD. The sensitivity of active chromatin in nuclei to micrococcal nuclease was suppressed by raising the ionic strength from 25 to 90 mM, indicating the occurrence of salt-induced condensation. The rate of sed...
متن کاملIsolation of transcriptionally active chromatin from mammalian nucleoli.
Nucleoli isolated from HeLa cells are functionally active but contain large amounts of RNA and proteins (RNA/DNA ratio 1:1; protein/DNA ratio 7:1). We have isolated from the nucleolus a DNA-protein complex that has the characteristics of nucleolar chromatin (RNA/DNA ratio less than 0.05:1; protein/DNA ratio 1.7:1). This nucleolar chromatin has most of the transcriptional activity of the intact ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1977
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/4.9.3155